Journal: iScience

Article Title: Borrelia-specific antibody profiles and complement deposition in joint fluid distinguish antibiotic-refractory from -responsive Lyme arthritis

doi: 10.1016/j.isci.2024.108804

Figure Lengend Snippet:

Article Snippet: Arp37 , Rockland , Cat# 000-001-C09.

Techniques: Recombinant, Produced, Generated, Software, Luminex

Journal: iScience

Article Title: Borrelia-specific antibody profiles and complement deposition in joint fluid distinguish antibiotic-refractory from -responsive Lyme arthritis

doi: 10.1016/j.isci.2024.108804

Figure Lengend Snippet:

Article Snippet: CRASP2 , Rockland , Cat# 000-001-C19.

Techniques: Recombinant, Produced, Generated, Software, Luminex

Journal: The Journal of Cell Biology

Article Title: Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore–microtubule dynamics

doi: 10.1083/jcb.201707160

Figure Lengend Snippet: Hec1 S69 is phosphorylated throughout mitosis. (A) Amino acid sequences of the human and PtK1 cell Hec1 N-terminal tail domain. Shown in yellow is the human peptide sequence that was used to generate the S69 phosphospecific antibody. The arrow points to S69 in the human sequence and the corresponding serine residue in the PtK1 sequence. Asterisks indicate all other mapped Aurora B kinase sites in the human Hec1 tail domain ( ; ). (B) Immunofluorescence images of HeLa cells stained with phosphospecific antibodies to Hec1 pS69. Depletion of Hec1 (bottom) results in loss of pS69 staining at kinetochores. Cells are also immunostained with antibody 9G3 (pan-Hec1 antibody) and an anticentromere antibody (ACA) derived from human calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, and telangiectasia (CREST) patient serum. (C) Immunofluorescence images of HeLa cells demonstrating kinetochore localization of pS69 during mitosis. Quantification is shown on the right. For each phase shown, ≥400 kinetochores from ≥30 cells were measured. (D) Immunofluorescence images of a HeLa cell stained with antibodies to pS69 and Mad2. For the cell shown, most chromosomes are aligned at the spindle equator, and one chromosome remains near a spindle pole (arrows). A schematic illustrating examples of pole-proximal chromosomes is shown on the left. (E) Immunofluorescence images of HeLa cells depleted of CENP-E to increase the number of pole-proximal chromosomes (arrows) and stained with Hec1 phosphospecific antibodies. Quantification is shown on the right from one representative experiment. n values are as follows: pS69, 20 polar kinetochores and 40 aligned kinetochores; pS55, 17 polar kinetochores and 57 aligned kinetochores; and pS44, 13 polar kinetochores and 29 aligned kinetochores. Error bars indicate SD. Bars: (B, C, and E) 10 µm; (D) 3 µm.

Article Snippet: Two rabbits were immunized with the Mad2 protein (Rockland Immunochemicals Inc), and antisera from the two injected rabbits were affinity purified against full-length Mad2 protein using a HiTrap-NHS column.

Techniques: Sequencing, Residue, Immunofluorescence, Staining, Derivative Assay

Journal: EBioMedicine

Article Title: Host glycosylation of immunoglobulins impairs the immune response to acute Lyme disease.

doi: 10.1016/j.ebiom.2024.104979

Figure Lengend Snippet: Fig. 2: Borrelia OspC- and VlsE-specific IgG combined with sialic acid abundance identifies acute Lyme disease patients. (a) Example of traditional detection of Borrelia burgdorferi (Bb) antigen-specific antibodies using a fluorescent reporter created with BioRender. (b) Example of GlycoLyme method detects a multiplexed sialic acid content and anti-IgG signal in a Bb antigen-specific antibody population created with BioRender. (c) Comparison of IgG abundance from non-Lyme-endemic healthy controls (NLEHC) n = 96, Lyme-endemic healthy controls (LEHC) n = 111, acute Lyme disease (ALD) n = 89, treated Lyme disease (TLD) n = 50, rheumatoid arthritis (RA) n = 20, lupus (L) n = 20, syphilis (S) n = 20, and fibromyalgia (F) n = 20. (d) Comparison of GlycoLyme [anti-OspC/VlsE IgG*SNA] scores from non-Lyme-endemic healthy controls (NLEHC) n = 96, Lyme-endemic healthy controls (LEHC) n = 111, acute Lyme disease (ALD) n = 89, treated Lyme disease (TLD) n = 50, rheumatoid arthritis (RA) n = 20, lupus (L) n = 20, syphilis (S) n = 20, and fibromyalgia (F) n = 20. (e) Receiver operating characteristic (ROC) curve compares ALD to non-ALD (NLEHC, LEHC, RA, L, S, F) with and without accounting for sialic acid abundance using the GlycoLyme method was analyzed with DeLong’s test to determine statical significance with ***p < 0.001. (f) Comparison of n = 35 GlycoLyme score positive ALD with matching TLD timepoint Bb-specific IgG abundance. (g) Comparison of n = 35 GlycoLyme score positive ALD with TLD time point Bb- specific immunoglobulin SNA fluorescence. Error bars present the mean ± standard deviation (SD). Statistical significance was determined using an ordinary one-way ANOVA with Tukey’s multiple comparisons test with ***p < 0.001 for figures (a and b), while paired two-tailed student’s t-test determined significance for figures (f and g) with ****p < 0.0001 and ns = not significant.

Article Snippet: The resulting UPLC fluorescent trace was analyzed with Empower v3.3.1 software, UPLC trace percent-area was combined with collected MSspectra to identify eluted peaks as described previously.32 Antigen-specific lectin multiplex assay High-binding 96-well plates (Nest Biotechnology, 514201) were incubated with the recombinantly expressed Borrelia burgdorferi (Bb) antigens: Outer Surface Protein C (OspC) protein (Rockland, 000-001-C11) and Variable Lipoprotein Surface-Exposed (VlsE) protein (Rockland, 000-001-C33).

Techniques: Comparison, Standard Deviation, Two Tailed Test